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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
ARP-811 is the LL58 cell line containing a persistent non-productive human immunodeficiency virus 1 IIIB (HIV-1 IIIB) infection. LL58 cells are an HIV-1 IIIB-infected subclone of X50-7, an EBV-transformed umbilical cord B lymphoblast line.
Growth Characteristics: Cells grow as a round, single-cell suspension with some clumping with a doubling time of 24 to 48 hours. Maintain cultures at 3 × 105 to 4 × 105 cells per mL.
Growth Medium: RPMI 1640 supplemented with 8% fetal bovine serum
ARP-811 is negative for Mycoplasma, bacteria and fungi. Each vial of ARP-811 contains approximately 8 × 106 cells in RPMI 1640 supplemented 20% fetal bovine serum and 8% dimethyl sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific information.
Dahl, K., et al. “Persistent Nonproductive Infection of Epstein-Barr Virus-Transformed Human B Lymphocytes by Human Immunodeficiency Virus Type I.” J. Virol. 64 (1990): 1771-1783.PubMed: 2157058. Dahl, K., K. Martin and G. Miller. “Differences among Human Immunodeficiency Virus Strains in Their Capacities to Induce Cytolysis or Persistent Infection of a Lymphoblastoid Cell Line Immortalized by Epstein-Barr Virus.” J. Virol. 61 (1987): 1602-1608. PubMed: 3033285.
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