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ARP-95 Human Immunodeficiency Virus 1 (HIV-1) Lymphadenopathy-Associated Virus (LAV)-Infected 8E5 Cells
(Cell Lines - Infected)

Price: All BEI Resources products are provided
at no cost to registered researchers.

Description:

Human Immunodeficiency Virus 1 (HIV-1) Lymphadenopathy-Associated Virus (LAV)-Infected 8E5 Cells

Organism:

Human Immunodeficiency Virus

Biosafety Level:

Availability Status:

In Stock

Store at:

-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer

Contributor:

T Folks

Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

ARP-95 is a subclone of lymphadenopathy-associated virus (LAV)-infected A3.01, a CD4⁺ CEM-derived human T-cell line.

ARP-95 is similar in morphology to other T-cell lines. The A3.01 parent cells were infected with LAV and selected by a series of 3 exposures to IUdR. Each 8E5/LAV subclone contains a single integrated copy of proviral DNA (no unintegrated DNA) directing synthesis of defective virus particles. Cells are CD4^-and secrete high levels of p24, but do not produce RT.

Note: Publications have shown that multiple passages of this cell line, particularly at high split ratios, can result in a loss of proviral DNA. See the second and third articles in the reference section for more information.

Growth Characteristics: When thawing, slowly dilute the cells with 37°C medium dropwise. Begin the culture at 2 × 10⁶ cells per mL, splitting the cells 24 hours later 1 × 10⁶ cells per mL. Passage the cells every three days thereafter to give a concentration of 1 × 10⁶cells per mL. Cells grow in single cell suspension with some clumping. ARP-95 has also been successfully grown in OPTI-MEM^® (Gibco™) containing 2.5% fetal bovine serum, 2.0 mM L-glutamine, 100 U per mL penicillin, 100 µg per mL streptomycin, and 0.5 µM ß-mercaptoethanol.

Growth Medium: RPMI 1640 supplemented with 10% fetal bovine serum

Each vial of ARP-95 contains approximately 3 × 10⁶ cells in RPMI 1640 supplemented with 10% fetal bovine serum and 7.5% DMSO. Please refer to the appropriate data sheet for lot-specific information.

Citations:

Acknowledgment for publications should read “The following reagent was obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH: Human Immunodeficiency Virus 1 (HIV-1) Lymphadenopathy-Associated Virus (LAV)-Infected 8E5 Cells, ARP-95, contributed by Dr. Thomas Folks."

Publications Citing this Reagent:

Busby, E., et al. “Instability of 8E5 Calibration Standard Revealed by Digital PCR Risks Inaccurate Quantification of HIV DNA in Clinical Samples by qPCR.” Sci. Rep. 7 (2017): 1209. PubMed:28446770.

Folks, T. M., et al. “Biological and Biochemical Characterization of a Cloned Leu-3-Cell Surviving Infection with the Acquired Immune Deficiency Syndrome Retrovirus.” J. Exp. Med. 164 (1986): 280-290.PubMed: 3014036.

Wilburn, K. M., et al. (2016). “Heterogeneous Loss of HIV Transcription and Proviral DNA from 8E5/LAV Lymphoblastic Leukemia Cells Revealed by RNA FISH:FLOW Analyses.” Retrovirology 13 (2016): 55. PubMed:27515378.

Restrictions On Use:

Scientists at for-profit institutions or who intend commercial use of this reagent must contact the Centers for Disease Control and Prevention Technology Transfer Office, Email: tto@cdc.gov, before the reagent can be released. Please specify the name and a description of the intended use of the reagent.

Product Name:

Human Immunodeficiency Virus 1 (HIV-1) Lymphadenopathy-Associated Virus (LAV)-Infected 8E5 Cells

Disclaimers:

You are authorized to use this product for research use only. It is not intended for human use.

Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC^® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC^® nor the U.S. Government warrants that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC^® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC^®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.

Citation:

Biosafety Level:

2 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.

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Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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Product Highlights for ARP-95

Product Sheet Information for ARP-95 Human Immunodeficiency Virus, Human Immunodeficiency Virus 1 (HIV-1) Lymphadenopathy-Associated Virus (LAV)-Infected 8E5 Cells, For research use only. Not for use in humans.

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