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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-1298 is a culture of U38 cells, a H938 derivative that contains stably integrated, silent copies of the human immunodeficiency virus 1 (HIV-1) long terminal repeat (LTR) promoter linked to the CAT gene.
The H938 cell line was generated by infection of H9 cells with a helper-free recombinant retroviral vector containing the HIV-1 LTR-CAT gene construct. H938 was selected in G418 under limiting dilution and is a sensitive indicator cell line for HIV-1 Tat. When infected by HIV-1, H938 produces high levels of chloramphenicol acetyl transferase (CAT). Morphology is lymphocyte-like. Contains LTR sequences to +80 in the R region. Contains the entire U3 region, but lacks U5 sequences.
Growth Characteristics: Split cells twice weekly at a ratio of 1:10. H938 cells are stable and do not need to be maintained in selection medium. If growth in selection medium is desired, propagation medium containing 700 µg per mL G418 should be used. Wash the thawed cells in propagation medium and centrifuge for 10 minutes at 1000 rpm before seeding the cells in a culture flask.
Growth Medium: RPMI 1640 supplemented with 10% fetal bovine serum
ARP-1298 is negative for bacteria, fungi and Mycoplasma.
Each vial of ARP-1298 contains approximately 6.8 × 106 cells in RPMI 1640 supplemented with 20% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific information.
Felber, B. K. and G. Pavlakis. “A Quantitative Bioassay for HIV-1 Based on Trans Activation. Science 239 (1988): 184-187.PubMed: 3422113.
Schwartz, S., et al. “Rapidly and Slowly Replicating Human Immunodeficiency Virus Type 1 Isolates can be Distinguished According to Target-Cell Tropism in T-Cell and Monocyte Cell Lines.” Proc. Natl. Acad. Sci.USA 86 (1989): 7200-7203. PubMed: 2789383.
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