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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-2187 expresses high levels of CD4 and are permissive to infection by human immunodeficiency virus 2 (HIV-2) and Simian immunodeficiency virus (SIV), but not HIV-1. ARP-2187 is derived from the parental U87MG cells, a human astroglioma cell line, transformed with a retroviral vector containing human CD4 and neomycin resistance genes.
Growth Characteristics: ARP-2187 has an epithelial-like morphology.
Growth Media: DMEM supplemented with 10% fetal bovine serum or 10% fetal calf serum
ARP-2187 is negative for Mycoplasma, bacteria and fungi.
Each vial of ARP-2187 contains approximately 1.2 × 107 cells per mL in DMEM supplemented with 50% fetal bovine serum and 10% dimethyl sulfoxide (DMSO).
Please refer to the attached file(s) for more information.
Dragic, T. and M. Alizon. “Different Requirements for Membrane Fusion Mediated by the Envelopes of Human Immunodeficiency Virus Types 1 and 2.” J. Virol. 67 (1993): 2355-2359.PubMed: 8095307. Chesebro, B., et al. “Failure of Human Immunodeficiency Virus Entry and Infection in CD4-Positive Human Brain and Skin Cells.” J. Virol. 64 (1990):215-221. PubMed: 2293663.
Ponten, J. and E. H. MacIntyre. “Long-Rerm Culture of Normal and Neoplastic Human Glia.” Acta Pathol. Microbiol. Scand. 74 (1968): 465-486. PubMed: 4313504.
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