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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-2884 is a cytopathic-sensitive clone of of the human T-cell acute lymphoblastic leukemia cell line CEM-TART that secretes the human immunodeficiency virus 1 (HIV-1) Rev and Tat proteins. ARP-2884 is especially suited to viability endpoint assays for determining anti-HIV activity with the replication defective HIV-1 strain HIV-1MC99Dtat-rev (ARP-1943).
Growth Characteristics: Cells grow in suspension, singly or in easily dissociated clumps. G418 is required to maintain the construct during passaging, but is not necessary for short-term antiviral assays. Split the cells as needed from 1 × 105 to 2 × 105 cells per mL to 1 × 106 to 2 × 106 cells per mL.
Growth Medium: RPMI 1640 medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine and 300 µg/mL G418
ARP-2884 is negative for bacteria, fungi and Mycoplasma.
Each vial of ARP-2884 contains approximately 1 × 107 cells in RPMI 1640 supplemented with 10% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific information.
Chen, H., et al. “Derivation of a Biologically Contained Replication System for Human Immunodeficiency Virus Type 1.” Proc. Natl. Acad. Sci. USA 89 (1992): 7678-7682. PubMed: 1502183.
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