Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

ARP-3317 are HOS cells, a human osteosarcoma cell line, expressing CD4 and CCR4.  The parental cell line is available as ARP-3313.  

ARP-3317 was generated by subcloning cDNA encoding CCR4 into the retroviral vector pBABE-puro. Amphotropic virus stocks were prepared by cotransfecting 293T cells with the resulting pBABE-puro construct, a VSV-G envelope expression vector, and pSV-gag-pol.?^-env^-. Supernatants were collected after 48 hours and used to infect HOS CD4+ Cells (ARP-3313). After another 48 hours, cells were selected in medium containing 1 µg per mL puromycin. ß-chemokine receptor expression can be maintained by culturing the cells in medium containing 0.5 µg per mL to 1.0 µg per mL puromycin.

ARP-3317 is part of a set of cell lines that express various ß-chemokine receptors, available as ARP-3312 through ARP-3319.

ARP-3317 is also referred to as HOS-CD4.CCR-4.

Growth Characteristics:  ARP-3317 grows as adherent, flat cells.  Thaw cells quickly at 37°C and immediately place them in 10 mL culture medium. Centrifuge at 400 × g to wash out DMSO, resuspend the cells in 10 mL fresh culture medium, and plate them onto a 10 cm² cell culture dish. Cells normally require a minimum of 3 to 4 days to recover, but should be checked daily to see if they need to be split. Cells split at 1:10 should become confluent after 3 days. Trypsinize and split at least twice per week; do not allow them to become over confluent.

Growth Medium:  DMEM supplemented with 10% fetal bovine serum and 0.5 µg per mL to 1.0 µg per mL puromycin

Note:  CD4 expression decreases with long-term (~ 4 months) culture in the absence of selection. Every few months, the CD4+ cells can be selected for by adding 40 µg per mL mycophenolic acid, 250 µg per mL xanthine, 13.5 µg per mL hypoxanthine and 10 mM HEPES to the growth medium. If desired, the cells can be maintained continuously in this CD4 selection medium.

ARP-3317 is negative for bacteria, fungi and Mycoplasma. 

Each vial of ARP-3317 contains frozen cells in DMEM supplemented with 20% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific information.