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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-459 expresses human CD4 protein on the cell surface and can be infected by most Human immunodeficiency virus (HIV) isolates. ARP-459 is derived from HeLa cells infected with a retroviral vector expressing CD4 and NeoR. Clones were selected for neomycin resistance and screened for the presence of surface CD4 via indirect immunofluorescence.
Foci of HIV-1 infected cells of ARP-459 can be detected by indirect immunoperoxidase or immunofluorescence using anti-HIV-1 serum or anti-HIV-1 monoclonal antibodies, or at a lower efficiency by syncytia formation. Cells can be grown in the presence of G418. Sensitivity of this lot for wild-type HIV is low. These cells are also infected with murine leukemia virus (MuLV) expressing the amphoteric MuLV envelope, and when infected by HIV, they can produce pseudotyped HIV particles with amphoteric MuLV envelope, which are capable of infecting some CD4-human and non-human cells.
Growth Characteristics: ARP-459 is a rapidly growing cell line with a variable epithelial morphology, and should be passaged by trypsinizing every 4 to 7 days at a ratio of 1:20 to 1:100. Cultures should be used when density is = 3 × 106 in a 25 cm2 flask.
Growth Medium: RPMI 1640 medium supplemented with 10% fetal bovine serum
ARP-459 is negative for Mycoplasma, bacteria and fungi.
Each vial of ARP-459 contains approximately 4.5 × 106 cells per mL in RPMI 1640 supplemented with 40% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific information.
Chesebro, B. and K. Wehrly. “Development of a Sensitive Quantitative Focal Assay for Human Immunodeficiency Virus Infectivity.” J. Virol. 62 (1988): 3779-3788. PubMed: 3047430. Chesebro, B., et al. “Failure of Human Immunodeficiency Virus Entry and Infection in CD4-Positive Human Brain and Skin Cells.” J. Virol. 64 (1990):215-221. PubMed: 2293663.
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