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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-501 is a Chinese hamster ovary (CHO) cell line that secretes soluble CD4 consisting of the entire extracellular region.
Growth Characteristics: ARP-501 has a medium growth rate with a flat, adherent culture. Maintain cells at about 2 × 106 . Feed with medium at least every 3 days. The cells also grow well in DMEM with fetal bovine serum, but continued production of CD4 must be monitored when this medium is used.
Growth Medium: Ham's F12 medium without hypoxanthine supplemented with 10% dialyzed, refiltered fetal bovine serum and 0.3 µM methotrexate
ARP-501 is negative for bacteria, Mycoplasma, yeast and mold.
Each vial of ARP-501 contains approximately 4 × 106 cells per mL in RPMI 1640 supplemented with 10% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the attached file(s) for additional information.
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For more information on the necessary compliance requirements associated with the biological materials provided by BEI Resources, please select this link: Compliance Requirements.