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NR-52287 consists of a crude preparation of cell lysate and supernatant from Chlorocebus aethiops kidney epithelial cells (Vero E6; ATCC® CRL-1586™) infected with severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate USA-WA1/2020 that was gamma-irradiated (5 × 106 RADs) on dry ice, followed by sonication.
SARS-CoV-2, isolate USA-WA1/2020 was isolated from an oropharyngeal swab from a patient with a respiratory illness who had recently returned from travel to the affected region of China and developed clinical disease (COVID-19) in January 2020 in Washington, USA.1
The complete genome of SARS-CoV-2, USA WA1/2020 has been sequenced (the isolate - GenBank: MN985325 and GISAID: EPI_ISL_404895 and after one passage in Vero cells - GenBank: MT020880). The complete genome of SARS-CoV-2, USA-WA1/2020 has been sequenced after four passages in Vero cells in collaboration with Database for Reference Grade Microbial Sequences (FDA-ARGOS; GenBank: MT246667).
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