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ARP-2861  Toxoplasma gondii, Strain RH(EP) Lambda (λ) Phage cDNA Library
(Libraries)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Toxoplasma gondii, Strain RH(EP) Lambda (λ) Phage cDNA Library
Organism: Toxoplasma gondii
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder 
Contributor: DS Roos, EO Kendall
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

ARP-2861 is a complementary DNA (cDNA) library prepared in a bacteriophage vector λDASH II (Stratagene) using mRNA from Toxoplasma gondii (T. gondii), strain RH(EP) (BEI Resources ARP-2859). A mixture of two independent cDNA syntheses were prepared using either AMV- or MuLV-reverse transcriptase, primed with a T-tailed XhoI oligonucleotide, directionally cloned from EcoRI to XhoI, packaged using Gigapack Gold (Stratagene), and amplified as plate lysate in Escherichia coli XL1-Blue according to the manufacturer's recommendations.

ARP-2861 contains approximately 2 × 106 independent recombinants with an average insert size of 500 to 4000 base pairs. Another version of this cDNA library, produced using mRNA from T. gondii, strain RH (BEI Resources ARP-1896) is available and may be more suitable for certain applications.

Each vial contains approximately 0.5 mL of transformed λ phage in suspension medium containing 7% dimethylsulfoxide (DMSO).
Citations: Acknowledgment for publications should read "The following reagent was provided by the NIH AIDS Reagent Program for distribution by BEI Resources, NIAID, NIH: Toxoplasma gondii, Strain RH(EP) Lambda (λ) Phage cDNA Library, ARP 2861, contributed by Dr. David S. Roos."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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