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ARP-2863  Toxoplasma gondii, Strain P(LK) Lambda (λ) Phage Genomic Library
(Libraries)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Toxoplasma gondii, Strain P(LK) Lambda (λ) Phage Genomic Library
Organism: Toxoplasma gondii
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder 
Contributor: DS Roos, EO Kendall
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

ARP-2863 is a complementary DNA library prepared in a bacteriophage vector λDASH II (Stratagene) using DNA from Toxoplasma gondii, strain P(LK) (BEI Resources ARP-2858) and partially digested with Sau3A. The partial Sau3A digest was cloned into the vector BamHI site, packaged using Gigapack Gold (Stratagene), and amplified as plate lysate in Escherichia coli XL1-Blue MRA(P2) cells according to the manufacturer's recommendations.

ARP-2863 contains approximately 6 × 105 independent recombinants, an average insert size of approximately 15000 base pairs, and 30,000 plaques, with a depth of coverage of approximately 5 genome equivalents.

Each vial contains approximately 0.5 mL of transformed lambda phage in suspension medium containing 7% dimethylsulfoxide (DMSO).
Citations: Acknowledgment for publications should read "The following reagent was provided by the NIH AIDS Reagent Program for distribution by BEI Resources, NIAID, NIH: Toxoplasma gondii, Strain P(LK) Lambda (λ) Phage Genomic Library, ARP-2863, contributed by Dr. David S. Roos."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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