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MRA-42  7G8, gDNA SmaI (BamHI Linearized) Library, Plasmodium falciparum
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Description: 7G8, gDNA SmaI (BamHI Linearized) Library
Organism: Plasmodium falciparum
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C
Contributor: DC Kaslow
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.

Plasmodium falciparum, strain 7G8 genomic DNA was digested with mung bean nuclease in 35% formamide under conditions resulting in genase activity. After blunt?end treatment, the fragments were cloned into the SmaI site of pUC13 using BamHI for linearization.

Kaslow, D. C. and S. Hill. "Cloning Metabolic Pathway Genes by Complementation in Escherichia coli. Isolation and Expression of Plasmodium falciparum Glucose Phosphate Isomerase." J. Biol. Chem. 265 (1990): 12337-12341. PubMed: 2197273.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, Strain 7G8, gDNA SmaI (BamHI Linearized) Library, MRA-42, contributed by David C. Kaslow."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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