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NR-40254   Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 12A9 (produced in vitro)
(Monoclonal Antibodies)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 12A9 (produced in vitro)
Organism: Crimean-Congo hemorrhagic fever virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: CS Schmaljohn
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Antibody Class: IgG1κ
Mouse monoclonal antibody prepared against the Crimean-Congo hemorrhagic fever virus (CCHFV) Pre-Gc glycoprotein was affinity purified from clone 12A9 hybridoma supernatant. Lot 61506733 was purified using protein G magnetic beads. Lot 70008695 was purified by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice immunized with protein A sepharose-bound CCHFV glycoprotein-antibody complexes as described by Bertolotti-Ciarlet et al.

This reagent is part of the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection.

Each vial of NR-40254 contains approximately 100 µL of purified monoclonal antibody in PBS.

NR-40254 is reactive in indirect immunofluorescence assays using Vero E6 cells infected with CCHFV, and neutralizes CCHFV in plaque reduction neutralization tests. Clone 12A9 antibody is also reported to function in ELISA and immunoprecipitation assays, to partially protect suckling mice from lethal CCHFV challenge, and to recognize an epitope that is conserved among some, but not all, CCHFV strains tested.
Citations: Acknowledgment for publications should read "The following reagent was obtained from the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection through BEI Resources, NIAID, NIH: Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 12A9 (produced in vitro), NR-40254."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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