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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-419  Vaccinia virus, Monoclonal Anti-Vaccinia (WR) L1R, Residues 1 to 185 (similar to VMC-4), (produced in vitro)
(Monoclonal Antibodies)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Monoclonal Anti-Vaccinia (WR) L1R, Residues 1 to 185 (similar to VMC-4), (produced in vitro)
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: GH Cohen, RJ Eisenberg
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Mouse monoclonal antibody to a recombinant form of the L1R protein [L1R(185t); residues 1 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from a mouse B cell hybridoma using ammonium sulfate precipitation and size exclusion chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-419 was purified from the same hybridoma as VMC-4. The specificity of the antibody was determined by reactivity to L1R(185t) by ELISA and confirmed by Western blot analysis using reducing and non-reducing conditions. VMC-4 does not neutralize the infectivity of the intracellular mature virus (IMV) form of vaccinia virus in BS-C-1 cells using an IMV plaque reduction assay.

Each vial contains approximately 1.0 mL of purified monoclonal antibody in 50 mM borate buffer (pH 8.0 +/- 0.2) containing 0.1 M sodium chloride and no preservatives.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Vaccinia Virus (WR) L1R Protein, Residues 1 to 185 (similar to VMC-4), (produced in vitro), NR-419."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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