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NR-844  Escherichia coli, Monoclonal Anti-Shiga Toxin 1 Subunit B (produced in vitro) (Similar to 13C4)
(Monoclonal Antibodies)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Monoclonal Anti-Shiga Toxin 1 Subunit B (produced in vitro) (Similar to 13C4)
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Antibody Class: IgG1κ
Mouse monoclonal antibody to the B subunit of Shiga-like toxin 1 from Escherichia coli (E. coli) H30 was purified using protein A (lot 4469046) or protein G (lot 61506759) affinity chromatography from supernatants obtained from a mouse hybridoma clonal cell line (ATCC ® CRL-1794™) that produces monoclonal antibody (MAb) 13C4. The hybridoma cell line was generated by the fusion of SP2/0 myeloma cells with immunized mouse splenocytes.

MAb 13C4 is specific to the B subunit of Shiga-like toxin 1 from E. coli H30 by standard Western blot analysis. MAb 13C4 binds both the denatured and native protein and immunoprecipitates intact labeled toxin. MAb 13C4 neutralizes the cytotoxicity of purified E. coli Shiga-like toxin 1 and Shigella dysenteriae Shiga toxin 1 as well as the Shiga-like toxic activities in crude cell extracts of Shigella flexneri, Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhimurium. MAb 13C4 has been successfully used in a colony blot assay for the detection of bacterial colonies which produce high levels of Shiga-like toxin 1.

Each vial of lot 4469046 contains approximately 50 µg of NR-844. Sodium azide (0.05%) was added to the preparation of purified monoclonal antibody as a preservative. Each vial of lot 61506759 contains approximately 10 µg of NR-844 in PBS and does not contain preservatives.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Shiga Toxin 1 Subunit B (produced in vitro) (Similar to 13C4), NR-844."
Publications Citing
this Reagent:
Karve, S. S. and A. A. Weiss. "Glycolipid Binding Preferences of Shiga Toxin Variants." PloS One 9 (2014): e101173. PubMed: 24983355.

Lu, X., et al. "A Poly-N-Acetylglucosamine-Shiga Toxin Broad-Spectrum Conjugate Vaccine for Shiga Toxin-Producing Escherichia coli." MBio. 5 (2014): e974. PubMed: 24667709.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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