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NR-845  Escherichia coli, Monoclonal Anti-Shiga-like Toxin 2 Subunit A (produced in vitro) (Similar to 11F11)
(Monoclonal Antibodies)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Monoclonal Anti-Shiga-like Toxin 2 Subunit A (produced in vitro) (Similar to 11F11)
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
NR-845 is no longer available.  Monoclonal Anti-Shiga-like Toxin 2 Subunit A (produced in vitro) (Similar to 11F11), is now available as NR-50336.

Antibody Class: IgG2aκ
Mouse monoclonal antibody to the A subunit of Shiga-like toxin 2 from Escherichia coli (E. coli) was purified by protein A affinity chromatography from supernatants obtained from a mouse hybridoma clonal cell line (ATCC ® CRL-1908™) that produces monoclonal antibody (MAb) 11F11. The hybridoma cell line was generated by the fusion of SP2/0 myeloma cells with immunized mouse splenocytes. MAb 11F11 immunoprecipitates intact labeled Shiga-like toxin 2 from E. coli as well as the isolated A subunit, but not the isolated B subunit. MAb 11F11 neutralizes the cytotoxicity of Shiga-like toxin 2 but not Shiga-like toxin 1 or Shiga toxin. MAb 11F11 has been successfully used in a colony ELISA for the detection of bacterial colonies which produce high levels of Shiga-like toxin 2.

Each vial contains approximately 50 micrograms of NR-845. Sodium azide (0.05%) was added to the preparation of purified monoclonal antibody as a preservative.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Shiga-like Toxin 2 Subunit A (produced in vitro) (Similar to 11F11), NR-845."
Publications Citing
this Reagent:
Karve, S. S. and A. A. Weiss. "Glycolipid Binding Preferences of Shiga Toxin Variants." PloS One 9 (2014): e101173. PubMed: 24983355.
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Escherichia coli Monoclonal Anti-Shiga-like Toxin 2, Subunit A, Clone 11F11 (produced in vitro)
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