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NR-846  Escherichia coli, Monoclonal Anti-Shiga Toxin 2 Subunit A (produced in vitro) (Similar to 11E10)
(Monoclonal Antibodies)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Monoclonal Anti-Shiga Toxin 2 Subunit A (produced in vitro) (Similar to 11E10)
Organism: Escherichia coli
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
NR-846 is no longer available. Monoclonal Anti-Shiga Toxin 2 Subunit A (produced in vitro) (Similar to 11E10), is now available as NR-45117.

Antibody Class: IgG1κ
Mouse monoclonal antibody to the A subunit of Shiga-like toxin 2 from Escherichia coli (E. coli) was purified by protein A affinity chromatography from supernatants obtained from a mouse hybridoma clonal cell line (ATCC ® CRL-1907™) that produces monoclonal antibody (MAb) 11E10. The hybridoma cell line was generated by the fusion of SP2/0 myeloma cells with immunized mouse splenocytes. MAb 11E10 immunoprecipitates intact labeled Shiga-like toxin 2 from E. coli as well as the isolated A subunit, but not the isolated B subunit. MAb 11E10 neutralizes the cytotoxicity of Shiga-like toxin 2 but not Shiga-like toxin 1 or Shiga toxin. MAb 11E10 has been successfully used in a colony ELISA for the detection of bacterial colonies which produce high levels of Shiga-like toxin 2.

Each vial contains approximately 50 micrograms of NR-846. Sodium azide (0.05%) was added to the preparation of purified monoclonal antibody as a preservative.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Shiga Toxin 2 Subunit A (produced in vitro) (Similar to 11E10), NR-846."
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Escherichia coli Monoclonal Anti–Shiga–Like Toxin 2 Subunit A, Clone 11E10 (produced in vitro)
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