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Product Name:
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CDC1551, Transposon Mutant 3334 (MT1363, Rv1321)
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Mycobacteriaceae, Mycobacterium
Species: Mycobacterium tuberculosis
Strain: CDC1551 (also referred to as CSU93 or Oshkosh)
Transposon Mutant: 3334 (MT1363, Rv1321)1,2,3
TN: NA1318
ID: Tn1363_394
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Additional Information:
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M. tuberculosis is a Gram-positive, rod-shaped aerobic bacterium. It is the causative agent of tuberculosis and is responsible for more morbidity in humans than any other bacterial disease.6
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Material Provided:
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Each vial contains approximately 0.7 mL of bacterial culture in Middlebrook 7H9 broth with ADC enrichment with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-18919 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Safety Precautions:
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BMBL recommends that practices with this agent include the use of respiratory protection and the implementation of specific procedures and use of specialized equipment to prevent and contain aerosols.
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Growth Conditions:
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Media:
Middlebrook 7H9 broth with ADC enrichment or equivalent
Middlebrook 7H10 agar with OADC enrichment or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic (with or without 5% CO2)
Propagation:
1. Keep vial frozen until ready for use; then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 2 to 6 weeks.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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3. Peterson, J. D., et al. “The Comprehensive Microbial Resource.” Nucleic Acids Res. 29 (2001): 123-125. PubMed: 11125067.
4. Valway, S. E., et al. “An Outbreak Involving Extensive Transmission of a Virulent Strain of Mycobacterium tuberculosis.” N. Engl. J. Med. 338 (1998): 633-639. PubMed: 9486991.
5. Lamichhane, G., et al. “A Postgenomic Method for Predicting Essential Genes at Subsaturation Levels of Mutagenesis: Application to Mycobacterium tuberculosis.” Proc. Natl. Acad. Sci. USA 100 (2003): 7213-7218. PubMed: 12775759.
6. Ducati, R. G., et al. “The Resumption of Consumption – A Review on Tuberculosis.” Mem. Inst. Oswaldo Cruz 101 (2006): 697-714. PubMed: 17160276.
7. Cole, S. T., et al. “Deciphering the Biology of Mycobacterium tuberculosis from the Complete Genome Sequence.” Nature 393 (1998): 537-544. PubMed: 9634230. Erratum in: Nature 396 (1998): 190-198.
8. de la Paz Santangelo, M., et al. “Mce3R, a TetR-Type Transcriptional Repressor, Controls the Expression of a Regulon Involved in Lipid Metabolism in Mycobacterium tuberculosis.” Microbiology 155 (2009): 2245-2255. PubMed: 19389781.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant 3334 (MT1363, Rv1321), NR-18919."
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Biosafety Level:
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3
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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