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NR-29400  Salmonella enterica subsp. enterica, 14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate 003/004_Kan
(Mutant Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Salmonella enterica subsp. enterica
Designations: 14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate 003/004_Kan
Biosafety Level: 2
Availability Status: In Stock
Store at: -80°C or colder
Contributor: H Andrews-Polymenis, M McClelland
Quantity limit per order for this item is 1.  This item can be ordered twice a year.  Orders over this limit will be sent to NIAID for approval before shipment.

The Salmonella enterica (S. enterica) subsp. enterica, strain 14028s (serovar Typhimurium) targeted single-gene deletion (SGD) mutant library contains a total of 3,773 individual genes deleted simultaneously across two collections of mutants differentiated by kanamycin or chloramphenicol resistance. The kanamycin-resistant mutant collection contains 3,517 mutants distributed among eleven 96-well plates. In these mutants, a single gene is replaced by a cassette conferring the kanamycin resistance gene, and includes 9 double mutants that contain both kanamycin and chloramphenicol cassettes. Deletions were confirmed by the depositor. The parent strain S. enterica subsp. enterica, strain 14028s is available from BEI Resources as NR-12154.

Genes were targeted for deletion by primers designed to preserve the first and last 30 bases of each deleted gene. Gene replacement followed a modified Lambda-Red technique, with an added T7 RNA polymerase promoter positioned in plasmid pCLF4 to generate a gene-specific transcript from the Salmonella genome directly downstream of each mutant.

Plate orientation and viability were confirmed for NR-29400.

Each inoculated well of the 96-well plate contains approximately 50 µL of culture in Luria Bertani (LB) broth containing 60 µg/mL kanamycin supplemented with 10% glycerol.

Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Salmonella enterica subsp. enterica, Strain 14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate 003/004_Kan, NR-29400."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Salmonella enterica subsp. enterica 14028s (Serovar Typhimurium)
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