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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-46563  Staphylococcus aureus subsp. aureus, JE2, Transposon Mutant NE20 (SAUSA300_0238)
(Mutant Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Staphylococcus aureus subsp. aureus
Designations: JE2, Transposon Mutant NE20 (SAUSA300_0238)
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: K Bayles, P Fey, NARSA
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Staphylococcus aureus (S. aureus) subsp. aureus, transposon mutant NE20 was derived from S. aureus subsp. aureus, strain JE2. Mutagenesis occurred through the use of the mariner-based transposon bursa aurealis resulting in an erythromycin-resistant deletion strain of JE2. S. aureus subsp. aureus, transposon mutant NE20 was created by disruption of SAUSA300_0238, which encodes for a BglG family transcriptional anti-terminator that prevents premature transcription termination.

S. aureus subsp. aureus, strain JE2 is a plasmid-cured derivative of strain LAC that was isolated in 2002 from a skin and soft tissue infection of an inmate in the Los Angeles County Jail in California, USA. Strain JE2 is a methicillin-resistant S. aureus (MRSA) strain and is a USA300 isolate. USA300 isolates have the same MLST profile (ST 8), SCCmec (subtype IV), agr group (I) and spa motif (MBQBLO) and typically carry the Panton-Valentine leukocidin (PVL) toxin genes and the arginine catabolic mobile element (ACME). USA300 is the most common cause of community-associated MRSA infection and an increasing cause of hospital-acquired infections.

Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth containing 5 µg/mL erythromycin supplemented with 10% glycerol.

The Nebraska Transposon Mutant Library (NTML) was constructed in the laboratories of Dr. Ken Bayles and Dr. Paul Fey at the University of Nebraska Medical Center. Additional information is available at the NTML website.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Staphylococcus aureus subsp. aureus, Strain JE2, Transposon Mutant NE20 (SAUSA300_0238), NR-46563."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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