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NR-48850  Staphylococcus aureus subsp. aureus, Nebraska Transposon Mutant Library (NTML) Genetic Toolbox
(Mutant Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Staphylococcus aureus subsp. aureus
Designations: Nebraska Transposon Mutant Library (NTML) Genetic Toolbox
Biosafety Level: 2
Availability Status: Discontinued (Please See Comments)
Store at: -60°C or colder
Contributor: NARSA, K Bayles
Comments:
NR-48850, Nebraska Transposon Mutant Library (NTML) Genetic Toolbox, is no longer available.  This item is available as BEI Resources NR-49947.  pJB38, the parent plasmid for the toolbox's plasmid constructs, is available for order separately as BEI Resources NR-49932.

The Center for Staphylococcal Research (CSR) at the University of Nebraska Medical Center has generated the Nebraska Transposon Mutant Library (NTML), a collection of sequence-defined transposon (Tn) insertion mutants of Staphylococcus aureus (S. aureus). To increase the functionality of the NTML, an allelic exchange system, the NTML Genetic Toolbox, was developed for the easy exchange of the transposons with either selectable markers or promoterless reporter genes. The selectable markers can be used to create multiple defined mutations within the S. aureus chromosome; whereas the reporter genes allow for the generation of single copy reporter constructs within any gene included in the NTML. The exchange plasmids are comprised of either a selectable marker or a reporter gene, flanked by the 5' and 3' ends of the bursa aurealis Tn.

All of the plasmids are in S. aureus, with the exception of pJB38 (the temperature-sensitive allelic exchange plasmid that is the parent plasmid of the toolbox's plasmid constructs) which is in Escherichia coli (E. coli) DH5a.

Each tube of recombinant S. aureus contains approximately 25 µL of bacterial culture in Tryptic Soy broth with 10 µg/mL chloramphenicol supplemented with 25% glycerol. The recombinant E. coli, DH5a tube contains approximately 25 µL of bacterial culture in Luria-Bertani (LB) broth with 100 µg/mL ampicillin supplemented with 25% glycerol.

Additional information about the genetic toolbox is available at the Nebraska Transposon Mutant Library website.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Nebraska Transposon Mutant Library (NTML) Genetic Toolbox, NR-48850."
Additional Information: NTML Genetic Toolbox
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-48501
Staphylococcus aureus subsp. aureus Nebraska Transposon Mutant Library (NTML) Screening Array
NR-49932
Nebraska Transposon Mutant Library (NTML) Genetic Toolbox Allelic Exchange Plasmid pJB38, Recombinant in Escherichia coli
NR-49947
Staphylococcus aureus subsp. aureus Nebraska Transposon Mutant Library (NTML) Genetic Toolbox
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