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NR-8034  Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plates 1-33
(Mutant Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Francisella tularensis subsp. novicida
Designations: "Two-Allele" Transposon Mutant Library, Plates 1-33
Biosafety Level: 2
Availability Status: In Stock
Contributor: C Manoil
Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Francisella tularensis subsp. novicida, strain U112 is excluded from Select Agent status. Please see CDC Select Agent Program, Notification of Exclusion.

NR-8034 consists of Plates 1-33 (BEI Resources NR-8035 through NR-8066 and NR-10484) of the "two-allele" 3050-member sublibrary. Plate 33 contains 18 strains which were not previously available on Plates 1-32. Eleven strains remain unavailable.

Each well of each 96-well plate contains approximately 0.25 mL of bacterial culture in 0.7X Tryptic Soy Broth containing 0.1% L-cysteine and supplemented with 5% glycerol. The broth also contains kanamycin (10 µg/mL) except for the strains in wells A04, A05 and A06 of Plate 33 (BEI Resources NR-10484). These 3 strains are erythromycin-resistant and were grown without antibiotic.

Note: Production in the 96-well format has a potential for cross-contamination. Individual mutants should be checked by the recipient prior to use.

Information about specific clones can be accessed through the Francisella Tularensis Genome Research homepage.

Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plates 1-33, NR-8034."
Additional Information: "Two-Allele" Transposon Mutant Library - Complete Map for Plates 1-33
Publications Citing
this Reagent:
Asare, R. and Y. Abu Kwaik. "Molecular Complexity Orchestrates Modulation of Phagosome Biogenesis and Escape to the Cytosol of Macrophages by Francisella tularensis." Environ. Microbiol. 13 (2011): 3310-3310. PubMed: 20482590.

Asare, R., et al. "Molecular Bases (sic) of Proliferation of Francisella tularensis in Arthropod Vectors." Environ. Microbiol. 12 (2011): 2587-2612. PubMed: 20482589.

Margolis, J. J., et al. "Francisella tularensis Subspecies novicida Chitinases and Sec Secretion System Contribute to Biofilm Formation on Chitin." Appl. Environ. Microbiol. 76 (2010): 596-608. PubMed: 19948864.
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

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