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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-8065  Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plate 31 (tnfn1_pw060510p03)
(Mutant Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Francisella tularensis subsp. novicida
Designations: "Two-Allele" Transposon Mutant Library, Plate 31 (tnfn1_pw060510p03)
Biosafety Level: 2
Availability Status: In Stock
Contributor: C Manoil
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Francisella tularensis subsp. novicida, strain U112 is excluded from Select Agent status. Please see CDC Select Agent Program, Notification of Exclusion.

NR-8065 represents Plate 31 (tnfn1_pw060510p03) of the "two-allele" 3050-member sublibrary. Strain tnfn1_pw060510p03q138 (Well F05) was not available due to quality issues at the time of release of Plate 31, but is now available on Plate 33 (BEI Resources NR-10484).

Each well of the 96-well plate contains bacterial culture in Tryptic Soy broth containing 0.1% L-cysteine and 10 µg/mL kanamycin supplemented with 5% glycerol.

Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual mutants should be purified (e.g. single colony isolation and purification using good microbiological practices) and verified prior to use. BEI Resources cannot confirm or validate any clone not identified on the plate information table.

Information about specific clones may also be accessed through the Francisella Tularensis Genome Research homepage.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plate 31 (tnfn1_pw060510p03), NR-8065."
Additional Information: "Two-Allele" Transposon Mutant Library - Complete Map for Plates 1-33
Publications Citing
this Reagent:
Jones, J. W. "Molecular Mechanisms of the Innate Immune Response to Francisella tularensis."  Diss. Stanford University, 2010.
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

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BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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