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NR-14867   Genomic DNA from Mycobacterium tuberculosis, Strain HN878
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Mycobacterium tuberculosis, Strain HN878
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit for this item is 2 per year. Orders over this limit will go to NIAID for approval before shipment.

NR-14867 is a preparation of genomic DNA extracted from a culture of Mycobacterium tuberculosis, strain HN878. The culture was grown to late log phase in glycerol-alanine-salts medium, and harvested by centrifugation. Cell lipids were removed and the delipidated cells were treated with lysozyme and RNase overnight followed by sodium dodecyl sulphate and Proteinase K. DNA was precipitated with isopropanol.

Each vial of NR-14867 contains approximately 100 µg of genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA buffer, pH 8).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Mycobacterium tuberculosis, Strain HN878, NR-14867."
Publications Citing
this Reagent:
Chakravorty, S., et al. "Rapid Detection of Fluoroquinolone-Resistant and Heteroresistant Mycobacterium tuberculosis by Use of Sloppy Molecular Beacons and Dual Melting-Temperature Codes in a Real-Time PCR Assay." J. Clin. Microbiol. 49 (2011): 932-940. PubMed: 21191047.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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