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NR-2519   Genomic DNA from Cryptosporidium parvum, Isolate Iowa
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Cryptosporidium parvum, Isolate Iowa
Organism: Cryptosporidium parvum
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -20°C
Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
Comments:
NR-2519, Genomic DNA from Cryptosporidium parvum, Isolate Iowa, is no longer available. 

Genomic DNA was isolated from a preparation of Cryptosporidium parvum, isolate Iowa.

Oocysts were produced in calves, concentrated from feces and purified using a standard protocol including salt flotation and bleach treatment. To minimize contamination, Nycodenz and CsCl gradient centrifugation was also performed. Oocysts were excysted in dilute taurocholic acid. Sporozoites and/or oocysts were pelleted and resuspended in lysis buffer prior to genomic DNA isolation.

Each vial contains approximately 1 microgram of genomic DNA in TE buffer.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Cryptosporidium parvum, Isolate Iowa, NR-2519."
Publications Citing
this Reagent:
Duggal, M. (2013). Detection and quantification of Cryptosporidium oocysts in environmental samples.

Shallom, S. J., et al. "A Species Independent Universal Bio-Detection Microarray for Pathogen Forensics and Phylogenetic Classification of Unknown Microorganisms." BMC Microbiol. 11 (2011): 132. PubMed: 21672191.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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