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NR-2520   Genomic DNA from Cryptosporidium hominis, Isolate TU502
(Nucleic Acids)
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at no cost to registered researchers.
Description: Genomic DNA from Cryptosporidium hominis, Isolate TU502
Organism: Cryptosporidium hominis
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -20°C
Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
NR-2520, Genomic DNA from Cryptosporidium hominis, Isolate TU502, is no longer available.

Genomic DNA was isolated from a preparation of Cryptosporidium hominis, isolate TU502.

Oocysts were produced in calves, concentrated from feces and purified using a standard protocol including salt flotation and bleach treatment. To minimize contamination, Nycodenz and CsCl gradient centrifugation was also performed. Oocysts were excysted in dilute taurocholic acid. Sporozoites and/or oocysts were pelleted and resuspended in lysis buffer prior to genomic DNA isolation.

Each vial contains approximately 100 ng of genomic DNA in TE buffer.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Cryptosporidium hominis, Isolate TU502, NR-2520."
Publications Citing
this Reagent:
Bouzid, M., et al. "Multi-Locus Analysis of Human Infective Cryptosporidium Species and Subtypes using Ten Novel Genetic Loci." BMC Microbiol. 10 (2010): 213. PubMed: 20696051.

Duggal, M. (2013). Detection and quantification of Cryptosporidium oocysts in environmental samples.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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