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NR-2634   Genomic DNA from Vaccinia Virus, Modified Vaccinia Ankara (MVA)
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Vaccinia Virus, Modified Vaccinia Ankara (MVA)
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: LS Wyatt, B Moss
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-2634, lot 62153956, genomic DNA was isolated from a preparation of cell lysate and supernatant from chicken embryo fibroblast cells (ATCC ® CRL-1590™) infected with vaccinia virus, MVA (BEI Resources NR-1).

NR-2634, lot 5075330, genomic DNA was isolated from a preparation of cell lysate and supernatant from hamster kidney cells (BHK-21; ATCC ® CCL-10™) infected with vaccinia virus, MVA (BEI Resources NR-1).

MVA is a highly attenuated strain of vaccinia virus and does not appear to replicate in most mammalian cells. The complete genomic sequence of MVA has been determined (GenBank: U94848).

NR-2634 has been qualified for PCR applications by amplification of a > 1,000 bp sequence.

Each vial of NR-2634, lot 62153956 contains 100 µL of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.

Each vial of NR-2634, lot 5075330, which is no longer available, contained a target amount of 1 × 108 copies of viral genomic DNA in TE buffer (pH 7.5) containing sodium azide.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Vaccinia Virus, Modified Vaccinia Ankara (MVA), NR-2634."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-1
Vaccinia virus Modified Vaccinia Ankara (MVA)
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