Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-2635   Genomic DNA from Vaccinia Virus, Lister (Elstree)
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Vaccinia Virus, Lister (Elstree)
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: ATCC®
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was isolated from a preparation of cell lysate and supernatant from African green monkey cells (Vero; ATCC ® CCL-81™) infected with vaccinia virus, Lister (Elstree; BEI Resources NR-51).

Vaccinia virus, Lister (Elstree) was originally isolated from the skin of a sheep. The Lister (Elstree) strain was widely used during the World Health Organization program on the eradication of smallpox. Although very effective, it induced rare but severe adverse effects. This was one of the reasons for the discontinuation of vaccination after eradication. The complete genomic sequence of vaccinia virus, Lister (Elstree) has been determined (GenBank: AY678276).

NR-2635 has been qualified for PCR applications by amplification of a sequence of at least 1000 bp. NR-2635 is not intended for use as a standard for quantitative PCR.

Each vial contains 100 µL of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Vaccinia Virus, Lister (Elstree), NR-2635."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-51
Vaccinia virus Lister (Elstree)
Return to Top


Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.


Back to My Search