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NR-2635   Genomic DNA from Vaccinia Virus, Lister (Elstree)
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Vaccinia Virus, Lister (Elstree)
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: ATCC®
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was isolated from a preparation of cell lysate and supernatant from African green monkey cells (Vero; ATCC ® CCL-81™) infected with vaccinia virus, Lister (Elstree; BEI Resources NR-51).

Vaccinia virus, Lister (Elstree) was originally isolated from the skin of a sheep. The Lister (Elstree) strain was widely used during the World Health Organization program on the eradication of smallpox. Although very effective, it induced rare but severe adverse effects. This was one of the reasons for the discontinuation of vaccination after eradication. The complete genomic sequence of vaccinia virus, Lister (Elstree) has been determined (GenBank: AY678276).

NR-2635 has been qualified for PCR applications by amplification of a sequence of at least 1000 bp. NR-2635 is not intended for use as a standard for quantitative PCR.

Each vial contains 100 µL of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Vaccinia Virus, Lister (Elstree), NR-2635."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Vaccinia virus Lister (Elstree)
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