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NR-2636   Genomic DNA from Vaccinia Virus, IHD
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Vaccinia Virus, IHD
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: ATCC®
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was isolated from a preparation of cell lysate and supernatant from cells infected with vaccinia virus, IHD (BEI Resources NR-52).

Lot 5100720 was prepared from infected Cercopithecus aethiops kidney epithelial cells (MA-104 Clone 1; ATCC ® CRL-2378.1™). Lot 63421352 was prepared from infected Macaca mulatta kidney epithelial cells (LLC-MK2 derivative; ATCC ® CCL-27.1™).

The IHD (International Health Division) strain was derived from the original New York City Board of Health (NYCBH) strain. The IHD strain was propagated for many passages by intracerebral inoculation in mice and has similar virulence to the Western Reserve (WR) strain. It is being used in a lethal respiratory infection model in mice to evaluate antiviral compounds. The coding sequences for several of the genes have been determined and are available in GenBank.

NR-2636 has been qualified for PCR applications by amplification of either an approximately 2600 bp sequence or an approximately 1100 bp sequence. NR-2636 is not intended for use as a standard for quantitative PCR.

Each vial of lot 5100720 contains a target amount of 1 ×108 copies of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.5) containing sodium azide. The actual number of copies per vial may vary from 10-fold lower to 10-fold higher.

Each vial of lot 63421352 contains 100 µL of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).

The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Vaccinia Virus, IHD, NR-2636."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Vaccinia virus IHD
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