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NR-2637   Genomic DNA from Vaccinia Virus, Lederle-Chorioallantoic
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Vaccinia Virus, Lederle-Chorioallantoic
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: ATCC®
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was isolated from a preparation of cell lysate and supernatant from African green monkey cells (ATCC ® CRL-2378.1™) infected with vaccinia virus, Lederle-Chorioallantoic (BEI Resources NR-53).

The Lederle-Chorioallantoic strain was derived from the New York City Board of Health (NYCBH) strain by passage in the chorioallantoic membrane of chicken embryos.

NR-2637 has been qualified for PCR applications by amplification of an approximately 2,600 bp sequence. NR-2637 is not intended for use as a standard for quantitative PCR.

Each vial contains a target amount of 1 X 10E8 copies of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.5) containing sodium azide. The actual number of copies per vial may vary from 10-fold lower to 10-fold higher. The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Vaccinia Virus, Lederle-Chorioallantoic, NR-2637."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-53
Vaccinia virus Lederle-Chorioallantoic
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