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NR-2644   Genomic DNA from Yersinia pestis, Strain A1122
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Yersinia pestis, Strain A1122
Organism: Yersinia pestis
Biosafety Level: 1
Availability Status: Temporarily Out of Stock (Please See Comments)
Store at: -20°C
Contributor: ATCC®
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

This item is currently in our production queue. Please allow ample time for distribution lots to be made available.


Genomic DNA was isolated from a preparation of Yersinia pestis (Y. pestis), strain A1122.

Y. pestis A1122 was isolated from a California ground squirrel (Spermophilus beecheyi) in California in 1939. It contains the 110 kb and the 19 kb plasmids, but lacks the 70 kb plasmid that is essential for virulence as well as the unstable pgm locus.

The presence of the 110 kb and the 19 kb plasmids in NR-2644 has been confirmed by PCR amplification of a virulence marker on each plasmid. NR-2644 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene as well as virulence marker sequences of approximately 1200 and 400 bp.

Each vial contains approximately 5 micrograms of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH approximately 7.4).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Yersinis pestis, Strain A1122, NR-2644."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-15
Yersinia pestis A1122
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