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Genomic DNA was extracted from a preparation of Escherichia coli (E. coli), strain EDL933, serotype O157:H7. The enterohemorrhagic E. coli (EHEC), strain EDL933 was isolated from raw hamburger meat implicated in a multi-state outbreak of hemorrhagic colitis in the United States in 1982.E. coli, strain EDL933 carries two plasmids, pO157 and pEDL933. Virulence-associated genes are located on both the chromosomal and plasmid DNA. The complete genome and pO157 sequences of E. coli, strain EDL933 are available (GenBank: NC_002655 and AF074613). The presence of plasmid pO157 and EHEC virulence markers in NR-2648 have been confirmed by PCR amplification. NR-2648 has been qualified for PCR applications by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene. Each vial contains 0.7 µg to 1.5 µg of bacterial genomic DNA in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 8.0). Lot 5107305 contains approximately 4 µg to 6 µg of bacterial genomic DNA in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 7.4). The vial should be centrifuged prior to opening.
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Information about permits is provided as a courtesy to BEI Resources customers. While we use reasonable efforts to include accurate and up-to-date information on this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the biological materials provided by BEI Resources, please select this link: Compliance Requirements.