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Genomic DNA was extracted from a preparation of
Escherichia coli (E. coli), strain EDL933, serotype O157:H7. The enterohemorrhagic E. coli (EHEC), strain EDL933 was isolated from raw hamburger meat
implicated in a multi-state outbreak of hemorrhagic colitis in the United
States in 1982.E. coli, strain EDL933 carries
two plasmids, pO157 and pEDL933. Virulence-associated
genes are located on both the chromosomal and plasmid DNA. The complete genome and pO157 sequences of E. coli, strain EDL933 are available (GenBank:
NC_002655 and AF074613). The presence of plasmid pO157 and EHEC
virulence markers in NR-2648 have been confirmed by PCR amplification. NR-2648 has been qualified for PCR applications
by amplification of approximately 1500 base pairs of the 16S ribosomal RNA gene. Each vial contains
0.7 µg to 1.5 µg
of bacterial genomic DNA in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 8.0). Lot
5107305 contains approximately 4 µg to 6 µg of bacterial genomic
DNA in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 7.4). The
vial should be centrifuged prior to opening.
If shipping to the U.S. State of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
When applying for permits and forms please:
Do NOT reference BEI Resources or ATCC Catalog Numbers in the Material Description
fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly
as they appear on your BEI Resources registration.
Information about permits is provided as a courtesy to BEI Resources customers.
While we use reasonable efforts to include accurate and up-to-date information on
this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the
biological materials provided by BEI Resources, please select this link: Compliance Requirements.
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