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NR-3071   Genomic DNA from Monkeypox Virus, WRAIR 7-61
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Genomic DNA from Monkeypox Virus, WRAIR 7-61
Organism: Monkeypox virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C
Contributor: ATCC®
Comments:
Quantity Limit per Order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was isolated from a preparation of cell lysate and supernatant from African green monkey cells (MA-104 Clone 1; ATCC ® CRL-2378.1™) infected with monkeypox virus, WRAIR 7-61 (BEI Resources NR-27).

Monkeypox virus, Walter Reed Army Institute of Research (WRAIR) 7-61 was isolated from a scab from a female cynomolgus monkey (Macaca fascicularis) that was observed with a poxvirus-like infection. The complete genomic sequence of monkeypox virus, WRAIR 7-61 has been determined (GenBank: AY603973).

NR-3071 has been qualified for PCR applications by amplification of an approximately 1,200 bp sequence. NR-3071 is not intended for use as a standard for quantitative PCR.

Each vial contains a target amount of 1 X 10E8 copies of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Monkeypox Virus, WRAIR 7-61, NR-3071."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-27
Monkeypox virus WRAIR 7-61
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