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NR-48853   Complementary DNA Synthesized from Schistosoma mansoni, Strain NMRI, Infected Mouse MicroRNA (chronic)
(Nucleic Acids)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Complementary DNA Synthesized from Schistosoma mansoni, Strain NMRI, Infected Mouse MicroRNA (chronic)
Organism: Schistosoma mansoni
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: MH Hsieh
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.

Complementary DNA (cDNA) was synthesized from microRNA (miRNA) extracted from pooled plasma of mice infected for 7 weeks with Schistosoma mansoni (S. mansoni), strain NMRI.

miRNAs are members of a rapidly growing class of small non-coding RNAs, 19 to 23 nucleotides in length, that are present in a highly stable cell-free form in body fluids of animals. miRNAs play an important role in disease and can serve as biomarkers of S. mansoni infection. S. mansoni miRNA was obtained from infected mouse plasma using the NucleoSpin® miRNA Plasma kit (Clontech) which is designed for the isolation of short RNA species from cell free blood plasma or serum. cDNA was synthesized from the miRNA with the Mir-X™ miRNA First-Strand Synthesis Kit (Clontech). In the synthesis reaction, miRNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and reverse transcriptase.

Each vial of NR-48853 contains approximately 1 µg of cDNA in DNase/RNase-free distilled water.
Citations: Acknowledgment for publications should read "The following reagent was provided by the NIAID Schistosomiasis Resource Center for distribution through BEI Resources, NIAID, NIH: Complementary DNA Synthesized from Schistosoma mansoni, Strain NMRI, Infected Mouse MicroRNA (chronic), NR-48853."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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