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Genomic RNA was extracted from a preparation of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero E6; ATCC ® CRL-1586™) infected with severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate Hong Kong/VM20001061/2020.
SARS-CoV-2, isolate Hong Kong/VM20001061/2020 was isolated from a nasopharyngeal aspirate and throat swab from an adult male patient on January 22, 2020 in Hong Kong. The complete genome of SARS-CoV-2, isolate Hong Kong/VM20001061/2020 has been sequenced (GISAID: EPI_ISL_412028).
Note: The SARS-Related Coronavirus 2, Isolate Hong Kong/VM20001061/2020, passage 6 (NR-52282 lot 70034432 or NR-52388 lot 70034679) sequence shows 6 sequence variants (5 with single nucleotide polymorphisms and one 27-base pair deletion in the ORF6 region) compared to the SARS-Related Coronavirus 2, Isolate Hong Kong/VM20001061/2020 (GISAID: EPI_ISL_412028). The variations were detected by whole genome sequencing completed at BEI Resources. Sequencing of an earlier passage of the virus is currently underway to confirm the variations. BEI Resources sequence is available at GenBank under accession number MT547814.
Each vial contains approximately 100 µL of viral genomic RNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic RNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.
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