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Plasmodium falciparum (P. falciparum), strain PfHDGFP is a transgenic clone of the 3D7 strain, created by tandem insertion of two copies of plasmid pHDGFP into the HRP III locus. The pHDGFP plasmid consists of a chimeric molecule of human dihydrofolate reductase (DHFR or HD) and green fluorescent protein (GFP) flanked by one set of gene control regions derived from 5’hrp3 and 3’hrp2 of P. falciparum. P. falciparum, strain 3D7 was originally isolated in the Netherlands.
P. falciparum, strain PfHDGFP stably expresses GFP and is reported to be resistant to methotrexate. The complete genome of P. falciparum, strain 3D7 has been sequenced (BioProject: PRJNA13173).
Note: This PfHDGFP clone is reported by MR4 users to be chloroquine resistant, and the PfCRT sequence from this clone at amino acids 74-76 is IET (unpublished). Likewise, genome fingerprinting of this clone did not match the P. falciparum 3D7 strain (BEI Resources MRA-102). Thus, the reported P. falciparum 3D7 parental lineage of PfHDGFP is in doubt.
Each vial contains approximately 0.5 mL of P. falciparum-infected human blood in Glycerolyte 57 solution (1:5).
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