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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-225  Toxoplasma gondii, ME49 (B7 clone)
(Parasitic Protozoa)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Toxoplasma gondii
Designations: ME49 (B7 clone)
Biosafety Level: 2
Availability Status: In Stock
Store at: -130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)
Contributor: LD Sibley
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Toxoplasma gondii (T. gondii), ME49 (originally isolated from a sheep in California and available as BEI Resources NR-362) was passed singly through a cat to obtain a line that was capable of producing oocysts, and then further cloned by limiting dilution to produce the B7 clone (also referred to as P and PLK strain).

Extensive testing of ME49, ME49 (B7 clone) and PTG (an independent clone of ME49) indicated that ME49 was effectively already a biological clone.

T. gondii, ME49 (B7 clone) is the Type II parental strain that was used in a genetic cross with the genotype Type III parental strain CTG ARA-SNF (also known as CTG ARA-AR/SNFR and CEP ARA-AR/SNFR and available as BEI Resources NR-10151) to test the feasibility of inter-strain crosses. Strain CTG ARA-SNF is the product of a genetic cross between singly resistant parental clones of the CTG (CEP) strain obtained by chemical mutagenesis.

T. gondii, ME49 (B7 clone) is commonly associated with AIDS. The whole genome shotgun sequence of ME49 (B7 clone) has been deposited in GenBank (ABPA01000000).

Note: This strain is also available as NR-10150 which has had additional characterization tests performed.

Each vial of NR-225 contains approximately 0.5 mL of culture in cryopreservative.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Toxoplasma gondii, ME49 (B7 Clone), NR-225."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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Derived from:  ATCC 50840
Toxoplasma gondii ME49 (B7 clone)
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