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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-42011  Trypanosoma brucei subsp. brucei, Lister 427 VSG 221 (TetR T7RNAP)
(Parasitic Protozoa)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Trypanosoma brucei subsp. brucei
Designations: Lister 427 VSG 221 (TetR T7RNAP)
Biosafety Level: 2
Availability Status: In Stock
Store at: -130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)
Contributor: G Cross
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Trypanosoma brucei (T. brucei) subsp. brucei, strain Lister 427 VSG 221 (TetR T7RNAP) was deposited to BEI Resources as a bloodstream form cell line that expresses the T. brucei variant surface glycoprotein (VSG) 221 (also referred to as MITat 1.2), T7 RNA polymerase (T7RNAP) and the tetracycline repressor (TetR) genes. The parental strain of Lister 427 VSG 221 (TetR T7RNAP), strain Lister 427 VSG 221 (BEI Resources NR-42009), is a single-antigen isolate of strain Lister 427. Strain Lister 427 is a virulent lab strain that was isolated in 1960 from a sheep in Uganda and transferred to the Lister Institute in London in 1961.

T. brucei subsp. brucei, strain Lister 427 VSG 221 (TetR T7RNAP) is a transgenic bloodstream form cell line that co-expresses the T7RNAP and TetR genes as well as VSG 221.

Each vial of NR-42011 contains approximately 0.5 mL of culture in 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Trypanosoma brucei subsp. brucei, Strain Lister 427 VSG 221 (TetR T7RNAP) (bloodstream form), NR-42011."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

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