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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-42012  Trypanosoma brucei subsp. brucei, Lister 427 29-13 (TetR T7RNAP) (procyclic form)
(Parasitic Protozoa)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Trypanosoma brucei subsp. brucei
Designations: Lister 427 29-13 (TetR T7RNAP) (procyclic form)
Biosafety Level: 2
Availability Status: In Stock
Store at: -130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)
Contributor: G Cross
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Trypanosoma brucei (T. brucei) subsp. brucei, strain Lister 427 29-13 (TetR T7RNAP) was deposited to BEI Resources as a procyclic form cell line that expresses the T7 RNA polymerase (T7RNAP) and the tetracycline repressor (TetR) genes. Strain Lister 427 29-13 (TetR T7RNAP) was derived by sequential stable transfection of the procyclic form of wild-type T. brucei subsp. brucei, strain Lister 427 (BEI Resources NR-42010). The parent strain Lister 427 is a virulent lab strain isolated in 1960 from a sheep in Uganda and transferred to the Lister Institute in London in 1961.

T. brucei subsp. brucei, strain Lister 427 29-13 (TetR T7RNAP), is a neomycin and hygromycin-resistant, transgenic procyclic form cell line, that co-expresses the T7RNAP and TetR.

Each vial of NR-42012 contains approximately 0.5 mL of culture in cryopreservative.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Trypanosoma brucei, Strain Lister 427 29-13 (TetR T7RNAP) (procyclic form), NR-42012."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

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