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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-49335  Toxoplasma gondii, Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC
(Parasitic Protozoa)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Toxoplasma gondii
Designations: Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC
Biosafety Level: 2
Availability Status: In Stock
Store at: -130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)
Contributor: J Boothroyd
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Toxoplasma gondii (T. gondii), strain Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC (also referred to as Pru A7 Δhpt::gra2-GFP::tub1-FLUC) was deposited to BEI Resources as a green-fluorescent-protein- and luciferase-expressing transgenic clone derived from the deletion mutant strain Prugniaud (Pru) Δhxgprt (also referred to as Pru Δhpt), which lacks the hxgprt (hypoxanthine-xanthine-guanine-phosphoribosyltransferase) gene. The original parent strain Prugniaud (Pru) is a Type II strain originally isolated in 1964 from a human with lethal congenital toxoplasmosis in Limoges, France. 

T. gondii, strain Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC was engineered by transfection of the Pru Δhxgprt strain with a plasmid expressing GFP and FLUC under the control of the Toxoplasma GRA2 and TUB1 promoters, respectively, resulting in constitutive expression of GFP and FLUC in both tachyzoite and bradyzoite stages of the parasite. Strain Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC has been used to study the role of bradyzoite-specific antigens in establishing infections

Each vial of NR-49335 contains approximately 0.5 mL of culture in cryopreservative [7.5% dimethylsulfoxide (DMSO)].
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Toxoplasma gondii, Strain Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC, NR-49335."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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