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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-51146  Toxoplasma gondii, ME49 TIR1-3FLAG
(Parasitic Protozoa)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Toxoplasma gondii
Designations: ME49 TIR1-3FLAG
Biosafety Level: 2
Availability Status: In Stock
Store at: -130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)
Contributor: LD Sibley
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Toxoplasma gondii (T. gondii), strain ME49 TIR1-3FLAG was deposited to BEI Resources as a transgenic strain, derived from the Type II luciferase-expressing strain ME49ΔHP::Luc. Strain ME49 TIR1-3FLAG was engineered by replacing the ku80 gene with the FLAG-tagged auxin receptor TIR1 using CRISPR-Cas9 technology.

T. gondii, strain ME49 TIR1-3FLAG is reported to stably express the auxin receptor TIR1 from Oryza sativa.

Each vial of NR-51146 contains approximately 0.5 mL of culture in cryopreservative [7.5% dimethylsulfoxide (DMSO)].
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Toxoplasma gondii, Strain ME49 TIR1-3FLAG, NR-51146.”
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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