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Product Name:
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NMRI, Schistosomes from Infected Female JAX Swiss Outbred Mice, Live
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Ownership statement:
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This reagent is the property of the U.S. Government.
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Manufacturer:
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Schistosomiasis Resource Center, Biomedical Research Institute, Rockville, Maryland, USA (NIH-NIAID Contract HHSN2700014l)
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Taxonomy:
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Flatworm Classification: Schistosomatidae, Schistosoma
Species: Schistosoma mansoni
Strain: NMRI
Host: JAX Swiss outbred mice
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Additional Information:
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S. mansoni is a species of trematode worm that causes the chronic parasitic disease schistosomiasis. Worldwide, more than 200 million people are infected and nearly 700 million are at risk, primarily in areas with poor sanitation that lack access to safe drinking water.
Infection occurs through contact with larval schistosomes (cercariae) that are released by freshwater snails. Upon exposure to infested water, these larvae penetrate human skin and travel through blood vessels to the liver where they mature and deposit eggs. Some of these eggs are then passed through human feces into water to re-infect the snail host and continue the parasite’s life cycle. Schistosome eggs that remain in the human body cause an immune response and damage to internal organs.
Collection of Schistosomes:
The schistosomes are collected at 6 to 7 weeks post-infection using warm complete medium (RPMI or DMEM-based) as perfusate, then transferred to fresh medium with a sterile brush, glass pipette or forceps.
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Material Provided:
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NR-61148 consists of schistosomes perfused from female JAX Swiss outbred mice (The Jackson Laboratory, Bar Harbor, Maine). Mice were exposed to ~ 200 cercariae of S. mansoni, strain NMRI and euthanized at 6 to 7 weeks post-infection.
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Packing/Storage:
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Schistosomes are placed in a 50 mL tube with complete medium and prepared for transport in an insulated shipper.3,4 Upon arrival, the schistosomes should be immediately placed at 37°C with 5% CO2.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Lewis, F. A., Personal Communication.
2. Lewis, F. A., et al. “Large-Scale Laboratory Maintenance of Schistosoma mansoni, with Observations on Three Schistosome/Snail Host Combinations.” J. Parasitol. 72 (1986): 813-829. PubMed: 3546654.
3. Acharya, S., A. A. Da’dara and P. J. Skelly. “Schistosome Immunomodulators.” PLoS Pathog. 17 (2021): e1010064. doi: 10.1371/journal.ppat.1010064. PubMed: 34969052.
4. Wang, J., R. Chen and J. J. Collins, III. “Systematically Improved in vitro Culture Conditions Reveal New Insights into the Reproductive Biology of the Human Parasite Schistosoma mansoni.” PLoS Biol. 17 (2019): e3000254. doi: 10.1371/journal.pbio.3000254. PubMed: 31067225.
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Citation:
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Acknowledgment for publications should read "The following reagent was provided by the Schistosomiasis Resource Center of the Biomedical Research Institute through NIH NIAID Contract HHSN272201700014I for distribution through BEI Resources, NIAID, NIH: Schistosoma mansoni, Strain NMRI, Schistosomes from Infected Female JAX Swiss Outbred Mice, Live, NR-61148."
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Biosafety Level:
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1
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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