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ARP-4690 is a universal lentiviral self-inactivated transducing vector that contains a chimeric 5' CMV-TATA enhancer/promoter site and a 3' bovine growth hormone polyA signal. The backbone is derived from pUC18 (of pNL4-3). This HIV-1 derived transducing vector contains the following HIV sequences: RU5, UTR without the major splice site, 720 base pair gag, 927 base pair env including the RRE, the two integration att sites and the 3'R.
NR-4690 was generated by combining the lentiviral transducing vector pTV, self-inactivating lentiviral vector pTYΔCT-CMVnlacZdl3'U3#1U5pA (see figures in plasmid and sequence file) and 5' splice site and env deletion env.dl.6 (see figures in plasmid and sequence file) with a NotI-EcoRI linker. Unique cloning sites on the linker that can be used for foreign gene insertion are: NotI, NheI, SalI, SmaI, BamHI, SacII, SpeI, EcoRI and KpnI.
Plasmids can be propagated in Escherichia coli (E. coli) STBL2 cells and grown at 37°C. Larger plasmids may benefit from growth at 30°C. This construct may also be grown in other competent cells. For additional details refer to the provided protocol for DNA transfection for production of recombinant lentiviruses, and the plasmid map and sequence files.
Each vial of ARP-4690 contains approximately 5 µg of dried purified DNA stabilized in DNAstable®PLUS. Please refer to the appropriate data sheet for lot-specific information.
Chang, L.-J., et al. “Efficacy and Safety Analyses of a Recombinant Human Immunodeficiency Virus Type 1 Derived Vector System.” Gene Ther. 6 (1999): 715-728. PubMed:10505094.
Iwakuma, T., Y. Cui Y and L.-J. Chang. “Self-Inactivating Lentiviral Vectors with U3 and U5 Mutations.” Virology 261 (1999): 120-132. PubMed:15750613.
Cui, Y., T. Iwakuma and L.-J. Chang. “Contributions of Viral Splice Sites and Cis-Regulatory Elements to Lentivirus Vector Function.” J. Virol 73 (1999): 6171-6176.PubMed: 10364378.
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