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MRA-103   Plasmid pCBM-BSD, for Transfection in Plasmodium falciparum
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Plasmid pCBM-BSD, for Transfection in Plasmodium falciparum
Organism: Plasmodium falciparum
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: DE Goldberg
Comments:
Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

The blasticidin-resistance gene (BSD) was amplified from the pcDNA6/V5-His plasmid (Invitrogen) using the primer pair 5'-GGAAGATGCATGCCAAGCCTTTGTCTCAAGAATCC ACCCTC-3' and 5'-GACGGGAAGCTTTGCTCCTCGGCCACGAAGTGC-3'. Amplified inserts were digested with NsiI and HindIII and used to replace the Toxoplasma gondii DHFR-TS in the pDT-Tg23 vector, yielding construct pCBM-BSD. The resulting size of the plasmid is 5.6 kilobases. The sequence has not been confirmed by BEI Resources. pCBM-BSD transfected P. falciparum 3D7 parasites confer resistance to blasticidin.

Each vial of MRA-103 contains approximately 20 µL of purified plasmid DNA in buffer at a concentration of 100 ng/20 µL.

Mamoun, C. B., et al. "A Set of Independent Selectable Markers for Transfection of the Human Malaria Parasite Plasmodium falciparum." Proc. Natl. Acad. Sci. USA 96 (1999): 8716-8720. PubMed: 10411941.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pCBM-BSD, for Transfection in Plasmodium falciparum, MRA-103, contributed by Daniel E. Goldberg."
Insert Size: 0.400
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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