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MRA-104   Plasmid pCBM-NEO, for Transfection in Plasmodium falciparum
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at no cost to registered researchers.
Description: Plasmid pCBM-NEO, for Transfection in Plasmodium falciparum
Organism: Plasmodium falciparum
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: DE Goldberg
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The neomycin phosphotransferase gene of transposon Tn5 was amplified from the pXG plasmid using the primer pair 5'-GGAAGATGCATGGATCGGCCATTGAACAAG-3' and 5'-GACGGGAAGCTTCTGTCTTTTTATTGCCGA-3'. Amplified inserts were digested with NsiI and HindIII and used to replace the Toxoplasma gondii DHFR-TS in the pDT-Tg23 vector, yielding construct pCBM-NEO. The resulting size of the plasmid is 6.0 kilobases. The sequence has not been confirmed by BEI Resources. pCBM-NEO transfected 3D7 parasite confer resistance to G418.

Each vial of MRA-104 contains approximately 20 µL of purified plasmid DNA in buffer at a concentration of 100 ng/20 µL.

Mamoun, C. B., et al. "A Set of Independent Selectable Markers for Transfection of the Human Malaria Parasite Plasmodium falciparum." Proc. Natl. Acad. Sci. USA 96 (1999): 8716-8720. PubMed: 10411941.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pCBM-NEO, for Transfection in Plasmodium falciparum, MRA-104, contributed by Daniel E. Goldberg."
Insert Size: 0.830
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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