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MRA-177   Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium falciparum
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium falciparum
Organism: Plasmodium falciparum
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: PA Zimmerman
Comments:

Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

The small subunit ribosomal RNA gene (18S rRNA gene; GenBank: AF145334) from Plasmodium falciparum was amplified from genomic DNA by nest 1 PCR primers and cloned into vector pCR2.1-TOPO (Invitrogen®).  The resulting plasmid (clone 8) may be used in PCR assays for the diagnosis of mixed species malaria infections.  MRA-177 was produced in One Shot® TOP10 chemically competent Escherichia coli (Invitrogen™) and extracted using a QIAGEN® QIAprep® Spin Miniprep Kit.

The resulting size of the plasmid is approximately 5100 base pairs and contains the genes required for ampicillin and kanamycin resistance.

Each vial of MRA-177 contains approximately 500 ng of plasmid DNA in TE buffer (10 mM Tris-HCl and 0.5 mM EDTA, pH 9).  The vial should be centrifuged prior to opening.

Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium falciparum, MRA-177, contributed by Peter A. Zimmerman.”
Insert Size: 1.200
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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