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MRA-179   Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium malariae
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Description: Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium malariae
Organism: Plasmodium malariae
Biosafety Level: 1
Availability Status: Temporarily Out of Stock (Please See Comments)
Store at: -20°C or colder
Contributor: PA Zimmerman
Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

This item is currently in our production queue. Please allow ample time for distribution lots to be made available.

The small subunit ribosomal RNA gene (18S rRNA gene; GenBank: AF145336) from Plasmodium malariae was amplified from genomic DNA by nest 1 PCR primers and cloned into vector pCR2.1-TOPO (Invitrogen®). The resulting plasmid (clone 34) may be used in PCR assays for the diagnosis of mixed species malaria infections. MRA-179 was produced in One Shot® TOP10 chemically competent Escherichia coli (Invitrogen™) and extracted using a QIAGEN® QIAprep® Spin Miniprep Kit.

The resulting size of the plasmid is approximately 5100 base pairs and contains the genes required for ampicillin and kanamycin resistance.

Each vial of MRA-179 contains approximately 500 ng of plasmid DNA in buffer. The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium malariae, MRA-179, contributed by Peter A. Zimmerman.”
Insert Size: 1.200
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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