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MRA-180   Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium ovale
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Description: Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium ovale
Organism: Plasmodium ovale
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: PA Zimmerman
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

MRA-180 is an Escherichia coli (E. coli) plasmid encoding the small subunit ribosomal RNA gene (18S rRNA gene; GenBank: AF145337) from Plasmodium ovale (P. ovale), strain Nigerian I. The small subunit ribosomal RNA gene was amplified from genomic DNA by nest 1 PCR primers and cloned into vector pCR2.1-TOPO (Invitrogen). The resulting plasmid (clone 54) may be used in PCR assays for the diagnosis of mixed species malaria infections. The recommended host for expression is E. coli TOP10. A multiple cloning site flanks the 18S rRNA open reading frame. Ampicillin and kanamycin (E. coli) were incorporated as selectable markers.

The resulting size of the plasmid is approximately 5000 base pairs.

Each vial contains approximately 0.2 µg of plasmid DNA in buffer. The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Diagnostic Plasmid Containing the Small Subunit Ribosomal RNA Gene (18S) from Plasmodium ovale, MRA-180, contributed by Peter A. Zimmerman.”
Insert Size: 1.100
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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