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MRA-84   Plasmid pD.-.D., for Transfection in Plasmodium berghei
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Plasmid pD.-.D., for Transfection in Plasmodium berghei
Organism: Plasmodium berghei
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: AP Waters
Comments:
Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Plasmodium berghei (P. berghei) expression vectors contain, next to the Toxoplasma gondii or P. berghei DHFR/TS selection cassette, the DHFR/TS 5' upstream and 3' downstream noncoding regions to drive expression of the gene of interest. Expression vectors were designed containing either a unique BamHI site or a linker sequence present at the junction of the empty non-coding regions so that any gene of interest can be cloned as a BamHI, BclI, Sau3A, BglII, XhoI or SalI fragment. The resulting size of the plasmid is 6.2 kilobases. The sequence has not been confirmed by BEI Resources.

Each vial of MRA-84 contains approximately 1 µg of plasmid DNA in buffer at a concentration of 1 µg/9.5 µL.

Waters, A. P., et al. "Transfection of Malaria Parasites." Methods 13 (1997): 134-147. PubMed: 9405197.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pD.-.D., for Transfection in Plasmodium berghei, MRA-84, contributed by Andrew P. Waters."
Insert Size: 3.5
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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