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MRA-97   Plasmid pDT.Tg23, for Transfection in Plasmodium falciparum
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Plasmid pDT.Tg23, for Transfection in Plasmodium falciparum
Organism: Plasmodium falciparum
Biosafety Level: 1
Availability Status: In Stock
Store at: 4°C
Contributor: Y Wu, TE Wellems
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

MRA-97 is a Plasmodium falciparum (P. falciparum) expression plasmid, pDT.Tg23, encoding Toxoplasma gondii dihydrofolate reductase-thymidylate synthase (tgdhfr/ts) under control of 5'HRP3 and 3'HRP2. Plasmid pDT.Tg23 was designed for transfection into the genome of P. falciparum parasites. The resulting size of the plasmid is 7.8 kilobases. The sequence has not been confirmed by BEI Resources.

Donald, R. G. and D. S. Roos. "Stable Molecular Transformation of Toxoplasma gondii: A Selectable Dihydrofolate Reductase-Thymidylate Synthase Marker Based on Drug-Resistance Mutations in Malaria." Proc. Natl. Acad. Sci. USA 90 (1993): 11703-11707. PubMed: 8265612.
Wu, Y., L. A. Kirkman and T. E. Wellems. "Transformation of Plasmodium falciparum Malaria Parasites by Homologous Integration of Plasmids that Confer Resistance to Pyrimethamine." Proc. Natl. Acad. Sci. USA 93 (1996): 1130-1134. PubMed: 8577727.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pDT.Tg23, for Transfection in Plasmodium falciparum, MRA-97, contributed by Yimin Wu and Thomas E. Wellems."
Insert Size: 4.700
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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